Name: Anti-ERK3/MAPK6 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; pig; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Mitogen-Activated Protein Kinase 6; Map Kinase 6; Mapk 6; Extracellular Signal-Regulated Kinase 3; Erk-3; Map Kinase Isoform P97; P97-Mapk (Gene ID:5597; UniProt:Q16659)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IHC; IP
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:128000.
    WB-In transfected HEK293 transiently expressing Mouse ERK3/Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1µg/ml.
    IHC-Paraffin embedded Human Heart. Recommended concentration: 10µg/ml.
    IF-Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts retrovirally transduced with MK5/ERK3 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11926. Western blots of the IP were labelled with rabbit anti-ERK3 serum or with mouse anti-MK5. An approx 100kDa of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

Anti-ERK3/MAPK6 antibody

Anti-ERK3/MAPK6 antibody

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