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Overview
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This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
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- Properties
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Overview
Immunoprecipitation analysis of 200ug extracts of THP-1 cells using 3ug Bcl-2 antibody (STJ27524). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (STJ27524) at a dilition of 1:1000.
Immunofluorescence analysis of U-2 OS cells using Bcl-2 Rabbit pAb (STJ27524) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Bcl-2 Rabbit pAb (STJ27524) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of BALB-3T3 cells using Bcl-2 Rabbit pAb (STJ27524) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human appendix using Bcl-2 Rabbit pAb (STJ27524) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using Bcl-2 antibody (STJ27524) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Anti-BCL2 antibody
Anti-BCL2 antibody
Catalog Number:
A001035959STJ
Mfr. No.:
STJ27524
Price:
$297
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