anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified

Overview
- Specifically detects intact AAV2 and AAV3 particles, both empty and full capsids. With AAV2 and AAV3 A20 recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. Not for WB.
  The A20 antibody is used as capture and detection antibody in the AAV2 Titration ELISA (PRATV).
  
  Please contact us at for specific academic pricing.
  
  Background
  
  For characterization of different stages of infection and very useful for the analysis of the AAV2 assembly process. A20 specifically reacts with AAV2 and AAV3, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. The antibody cannot be used for immunoblotting.
  Epitope mapping experiments (Wobus et al. 2000) identified four immunoreactive (discontinous) regions. The major reaction was attributed to sequence aa 369 to aa378 of AAV2 capsids. The antibody is also useful for neutralizing experiments.
  
  Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).
  Moskalenko, M. et al. Epitope Mapping of Human Anti-Adeno-Associated Virus Type 2 Neutralizing Antibodies: Implications for Gene Therapy and Virus Structure. Jounal Virol. 74, 1761–1766 (2000).
  
  More Details
  
  AAV Antibodies
Properties
- Host
  
  mouse
  
  Antibody Type
  
  monoclonal
  
  Isotype
  
  IgG3
  
  Reactivity
  
  virus (AAV2, AAV3)
  
  Clone
  
  A20
  
  Immunogen
  
  AAV2 capsids
  
  Purification
  
  protein A affinity chromatography
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
  
  Storage
  
  short term at 2-8°C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
  
  * Research use only

Applications

Application

Affinity Chromatography; ELISA; ICC/IF; IP; Neutralization Assay

Application	Dilutions
Affinity Chromatography	assay dependent
Dot Blot	1:500
ELISA	assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	1:20
Immunoprecipitation (IP)	1:5
Neutralization Assay	assay dependent

Application	Species	Publication
Affinity Chromatography	AAV2 AAV2.7m8	PMID: S104784771930259X
ELISA	AAV2 AAV3	PMID: 24299301
ICC/IF	AAV2	PMID: PMC3676809
IP	AAV2	PMID: 23015698
Neutralization Assay	AAV2	PMID: 21917944

Reference
- Bennett, A. et al. Structure comparison of the chimeric AAV2.7m8 vector with parental AAV2. J. Struct. Biol. 209, 107433 (2020).
  Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1-12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212-222 (2014).
  Jin, L.-F. et al. Ultrasound Targeted Microbubble Destruction Stimulates Cellular Endocytosis in Facilitation of Adeno-Associated Virus Delivery. Int. J. Mol. Sci 14, 9737-9750 (2013).
  Naumer, M. et al. Properties of the Adeno-Associated Virus Assembly-Activating Protein. J. Virol. 86, 13038-13048 (2012).
  Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686-12697 (2011).
  Moskalenko, M. et al. Epitope mapping of Human Anti-Adeno-Associated Virus Type 2 Neutralizing Antibodies: Implications for Gene Therapy and Virus Structure. Jounal Virol. 74, 1761-1766 (2000).
  Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281-93 (2000)
  Grimm, D. et al. Titration of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant AAV-2. Gene Ther. 6, 1322-30 (1999).
  Bartlett, J. S., Kleinschmidt, J., Boucher, R. C. & Samulski, R. J. Targeted adeno-associated virus vector transduction of nonpermissive cells mediated by a bispecific F(abâgamma)2 antibody. Nat. Biotechnol. 17, 181-6 (1999).
  Wistuba, A. et al. Subcellular Compartmentalization of Adeno-Associated Virus Type 2 Assembly. J. Virol. 71, 1341-1352 (1997).
  Wistuba, A., Weger, S., Kern, A., Rgen, J. & Kleinschmidt, A. Intermediates of Adeno-Associated Virus Type 2 Assembly: Identification of Soluble Complexes Containing Rep and Cap Proteins. J. Virol. 69, 5311-5319 (1995).

Datasheet

anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified

anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified

Background

More Details

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