AAV2 ELISA Control

Overview
- The AAV2 ELISA Control consists of fully assembled, empty AAV2 capsids. It represents the serotype-specific conformational epitopes and is therefore a reliable positive control in detection and quantification assays based on AAV antibodies detecting the conformational epitopes, such as ELISA.
  
  Please contact us at for specific academic pricing.
  
  Background
  
  The AAV2 ELISA Control consists of fully assembled, empty AAV2 capsids. The concentration is lot specific.
  
  The AAV2 ELISA Control can be used as a positive control with PROGEN’s AAV2 Titration ELISA (PRATV & PRAAV2R). If you require different lots of the AAV2 ELISA Control and the Kit Control included in your PROGEN AAV2 Titration ELISA, please enquire to check availability.
  
  Reading of the AAV2 ELISA Control from the standard curve (i.e. Kit Control, included in PROGEN’s AAV2 Titration ELISA) is influenced by inter- and intra-assay, but also by inter-lab variances. Therefore, it is recommended that each laboratory determines its own acceptable range of recovery.
  
  The AAV2 ELISA Control has been calibrated on the ATCC AAV2 reference standard material (RSM, VR-1616). For further information, please see the publication by Lock et al., 2010.
  Lock M, McGorray S, et al. Characterization of a recombinant Adeno-Associated Virus Type 2 reference standard material; Hum Gene Therapy 21:1273-1285 (2010).
  
  Grimm D, Kern A, Pawlita M, Ferrari F, Samulski R and Kleinschmidt J. Titration of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant AAV-2. Gene Therapy 6:132-1330 (1999).
  
  More Details
  
  AAV Titration ELISA Kits
Properties
- Formulation
  
  Lyophilized, empty AAV2 capsids. Reconstitute in 500 μl ASSB 1x (provided with PROGEN’s AAV2 Titration ELISA), incubate 5 min at RT and mix by rolling 5 min. Avoid vortexing! The final solution contains stabilizing protein, phenol red and ASSB 1x buffer.
  
  Storage
  
  store at 2-8°C
  
  Concentration
  
  1.7E+09 – 2.8E+09 capsids/ml after reconstitution in 500 µl ASSB 1x (please find the lot-specific concentration on the CoA and on the vial)
  
  Stability
  
  4 weeks at 2-8°C after reconstitution in ASSB 1x
  
  * Research use only

Applications

Application

ELISA

Application	Dilutions
ELISA	As a positive control in ELISA, a 1:4 dilution in ASSB 1x (provided with our AAV2 Titration ELISA) and analysis at least in duplicates is recommended.

Datasheet

Alternative Products

Catalog Number	Product Name	Format	Price
PRAAV1-C	AAV1 ELISA Control	1 vial (0.6E+09 – 1.0E+09 capsids)	$297
PRAAV3-C	AAV3 ELISA Control	1 vial (5.5E+08 – 9.5E+08 capsids)	$297
PRAAV5-C	AAV5 ELISA Control	1 vial (3.9E+09 – 6.5E+09 capsids)	$297
PRAAV6-C	AAV6 ELISA Control	1 vial (1.0E+09 – 1.6E+09 capsids)	$297
PRAAV8-C	AAV8 ELISA Control	1 vial (2.8E+08 – 4.7E+08 capsids)	$297
PRAAV9-C	AAV9 ELISA Control	1 vial (4.5E+08 – 7.5E+08 capsids)	$297