Prime-Fect In Vivo Kit - Primary Cell Transfection Reagent for siRNA and pDNA Delivery

Prime-Fect In Vivo kit contains Prime-Fect reagent at 1 mg/mL and Trans-Booster at 0.4 mg/mL.

The Prime-Fect Kit is a highly effective transfection reagent combination optimized for attachment-dependent cells. The kit components are derived from synthetic amphiphilic polymers tailored for pDNA and siRNA delivery after extensive testing of polymer libraries. It can undergo multivalent interactions with nucleic acids and encapsulating them into nanoparticles (~100 nm) appropriate for effective cellular uptake. This interaction occurs in aqueous buffer, obviating the need for organic solvents during preparation. The Prime-Fect Kit components are non-integrating carriers, so that the genetic make-up of host cells is not altered after the treatment. These materials have been tested and found effective in primary cells and in certain cell lines. As with all transfection reagents, formulations of Prime-Fect Kit with polynucleotides may need to be optimized for specific cell types and transfection conditions

Benefits
• High transfection efficiency in the presence of serum.
• Effective delivery of pDNA molecules via a simple protocol that is ideal for scale-up and automation.
• Minimal toxicity compared to other commercial reagents, reducing adverse effects on highly sensitive human cells.
• Non-integrating transfection reagent eliminates unwanted effects due to host genome integration.
• Possibility of using the same transfection reagent in animal models, leading to harmonized studies.

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Background

Prime-Fect is a highly effective transfection reagent for attachment-dependent primary cells. Prime-Fect is a synthetic amphiphilic polymer that is tailored primarily for DNA delivery to a wide range of primary cells. It is capable of undergoing multivalent interactions with polynucleotides and encapsulating co-incubated polynucleotides into 100-200 nm particles with a net positive charge. The complexation between the polynucleotides and the Prime-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. Prime-Fect is a nonintegrating carrier, so that the genetic make-up of host cells is not altered after treatment with the transfection reagent. Prime-Fect has been tested and also found effective for siRNA delivery to different types of attachment dependent cells. As with all transfection reagents, formulations of Prime-Fect with DNA or RNA may need to be optimized for specific cell types and transfection conditions.