mRNA-Fect In Vivo - mRNA Transfection Reagent for Animal Models

Transfection reagents for mRNA delivery in both in vitro and in vivo models. mRNA-Fect In Vivo Kit is recommended for better efficacy in hard-to-transfect cell types.

The mRNA-Fect In Vivo is a highly effective transfection reagent optimized for mRNA delivery in preclinical animal models. It is a high concentration formulation of mRNA-Fect to allow high amounts of mRNA delivery typical of preclinical models. The reagent is based on synthetic amphiphilic polymerstailored primarily for mRNA delivery after extensive testing of polymer libraries. The reagent can undergo multivalent interactions with mRNA, encapsulating it into nanoparticles (~100 nm) appropriate for effective cellular uptake. This interaction occurs in aqueous buffer, obviating the need for organic solvents during complex preparation. The mRNA-Fect In Vivo is a non-integrating carrier, so that the genetic make-up of host cells is not altered after the treatment. The transfection reagent has been tested and found effective for plasmid DNA and siRNA delivery as well in certain cell types. As with all transfection reagents, formulations of mRNA-Fect In Vivo with polynucleotides may need to be optimized for specific applications and transfection conditions.

Please contact us at for specific academic pricing.

Background

The mRNA-Fect reagent is a highly effective transfection reagent optimized for mRNA delivery to both attachment-dependent and suspension-growing cells. mRNA-Fect reagent is a synthetic amphiphilic polymer that is tailored primarily for mRNA delivery after extensive testing of amphiphilic polymer libraries. It is capable of undergoing multivalent interactions with polynucleotides and encapsulating coincubated mRNA into approximately 100 nm particles with a net positive charge. The complexation between the mRNA and the mRNA-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. The mRNA-Fect is a non-integrating carrier, so that the genetic make-up of host cells is not altered after treatment with this reagent. The mRNA-Fect has been tested and found effective for plasmid DNA and siRNA delivery as well in certain cell types. As with all transfection reagents, formulations of mRNA-Fect with polynucleotides may need to be optimized for specific cell types and transfection conditions.