ADC Control Kit with VC-PAB Linker

This kit is designed to label any IgG antibody with a VC-PAB linker. The final labeled product can be used as a negative control for any ADC with VC-PAB linker via thiol labeling chemistry. The preparation is essentially the same as standard ADC preparation with VC-PAB linker. The user supplies the antibody and the kit includes maleimide-activated VC-PAB-OH, which can be coupled directly to the antibody after reduction through alkylation in a single step (a method developed by Seattle Genetics: Sun et al. 2005, Bioconjugate Chem. 16, 1282-1290). The kit provides materials to then purify the ADC control by removing any unreacted linkers.

This kit provides materials to conjugate 1-3 mg of one (CM11429x1) or three (CM11429x3) antibody samples (IgG).

If you have a smaller amount of IgG, a non-IgG type antibody, or other customization needs, please select the customization radio button and/or fill out the information above.

• Linker Information
Name: Mc-VC-PAB-OH
CAS number: 159857-80-4
Chemical formula: C28H40N6O7
MW: 572.7 Da

• Requirement for Antibody
Preferably >90% pure by gel electrophoresis
Total amount: 1-3 mg protein content measured by UV

• Key Features of this PerKit™
Offers a simple and easy way to prepare negative control ADC with cathepsin B cleavable VC-PAB linkage (Ref. Doronina et al. 2008, Bioconjugate Chem. 19, 1960-1963)
Stable linkage
Fast and easy preparation: 6 h preparation with <2 h hands-on time
All reagents and supplies included for preparation and purification
More than 95% labeled products (free of unreacted linker)

Please contact us at for specific academic pricing.

More Details