Protein Mertansine (DM1) Conjugation Kit

This personalized conjugation kit to work with any protein with a MW over 20 KDa. The customer supplies its own unmodified protein. Using the kit components, the customer converts their protein to a maelimide-protein via SMCC (succinimidyl trans-4-(maleimidylmethyl)cyclohexane-1-carboxylate) linker, followed by reaction of the maleimide-protein with Mertansine (DM1) to generate the conjugates (a method developed by Immunogen). One step purification typically provides the resulting protein-DM1 at greater than 95% purity.

This kit provides materials to conjugate 6.67 to 20 nmol of one (CM11414x1) or three protein samples (CM11414x3).

If you have a smaller amount of protein, a protein with MW below 20KDa, or other customization needs, please select the customization radio button and/or fill out the information above.

• Requirement for protein:
Amount: 6.67-20 nmol
MW: >20 KDa
Preferably >90% pure by gel electrophoresis or SEC HPLC analysis
Protein has some lysine residues

• Drug Information
Name: Mertansine (DM1)
CAS number: 139504-50-0
Mechanism of action: Inhibits cell division by blocking the polymerisation of tubulin

• Key Features of this PerKit™
Offers a simple and easy way to label protein with DM1 with minimum exposure to the toxin
Stable linkage
Fast and easy preparation: 6 h preparation and <2 h hands-on time
All reagents and supplies included for preparation and purification
Loading is optimized based on the MW of the protein

Please contact us at for specific academic pricing.

More Details