Ac-DEVD-pNA

- Overview
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    Background
    
    Ac-DEVD-pNA is a colorimetric substrate for caspase-3 (Km=9.7µM) and caspases-1, -4, -6, and -7 [1]. Ac is the acetyl group and pNA is p-nitroanilide. Sequence is based on PARP (poly(ADPribose) polymerase) cleavage site Asp216 for caspase-3. In apoptosis, activated caspase-3 cleaves substrates such as poly(ADP-ribose) polymerase that targets at the amino acid sequence DEVD [2]. Cleavage is monitored colorimetrically at 405nm. Ac-DEVD-pNA can be used to identify and quantify caspase-3 activity in apoptotic cell lysates.
    
    1. Talanian, R.V.,Quinlan, C.,Trautz, S., et al. Substrate specificities of caspase family proteases. The Journal of Biological Chemisty 272(15), 9677-9682 (1997).
    2. Lazebnik, Y.A.,Kaufmann, S.H.,Desnoyers, S., et al. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 371, 346-347 (1994).
- Properties
  - Alternative Name
    
    7-(2-carboxyethyl)-4-(carboxymethyl)-10-isopropyl-13-((4-nitrophenyl)carbamoyl)-2,5,8,11-tetraoxo-3,6,9,12-tetraazapentadecan-15-oic acid
    
    CAS Number
    
    189950-66-1
    
    Molecular Formula
    
    C26H34N6O13
    
    Molecular Weight
    
    638.58
    
    Appearance
    
    A solid
    
    Purity
    
    98.00%
    
    Solubility
    
    ≥2.41 mg/mL in EtOH with ultrasonic; ≥6.39 mg/mL in H2O; ≥9.94 mg/mL in DMSO
    
    Storage
    
    Store at -20°C
    
    SMILES
    
    O=C(O)CCC(NC(C(NC(C)=O)CC(O)=O)=O)C(NC(C(C)C)C(NC(CC(O)=O)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)=O)=O
    
    * For Research Use Only