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Overview
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AacCas12b (previously known as AacC2c1) is a DNA endonuclease mediated by tracerRNA and crRNA (or sgRNA), which can specifically cleave the dsDNA target in a PAM (TTN)-dependent manner, generating DSBs, and cleave ssDNA target in a PAM-independent manner. In addition, both dsDNA and ssDNA targets can trigger the trans-cleavage activity of AacCas12b, that is, when Cas12b binds sgRNA and target DNA to form a ternary complex, the trans-cleavage activity of Cas12b will be released to non-specifically cleave ssDNA sequences in the reaction system. AacCas12b is derived from Alicyclobacillus acidoterrestris, and the optimal reaction temperature is 48°C for AacCas12b. In addition, AacCas12b can be used not only for specific cleavage of dsDNA in vitro, but also for rapid detection of target nucleic acid. (for details, please refer to PMID: 31532637).
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