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Overview
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Un1Cas12f1 (previously known as Un1Cas14a) is an endonuclease that specifically binds and cleaves ssDNA target under the guidance of tracrRNA and crRNA (or sgRNA alone), and does not require a PAM site. In addition, Un1Cas12f1 can also specifically cleave dsDNA target in a PAM-dependent manner, causing DSBs and generating sticky ends. Similar to other Cas12 family proteins, Un1Cas12f1 protein also has the trans-cleavage activity against ssDNA, and both dsDNA or ssDNA targets can trigger its trans-cleavage activity, that is, when Un1Cas12f1 binds sgRNA and target DNA to form a ternary complex, it releases trans-cleavage activity to non-specifically cleave ssDNA sequences in the reaction system.
Compared with other Cas proteins, Cas12f1 protein is generally smaller (400-700 AA). Similar to Cas12a and Cas12b, Cas12f can also be used to develop CRISPR-Dx systems for rapid detection of nucleic acids (For details, please refer to PMID: 30337455).Please contact us at for specific academic pricing.
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- Properties
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Overview