3,3,5,5-Tetramethylbenzidine - Amerigo Scientific

- Overview
  - TMB is commonly used as a chromogenic substrate for horse radish peroxidase for detection purposes in assay formats, such as enzyme-linked immunosorbent assays (ELISAs). TMB is a colourless solution that changes to blue upon oxidation. In the ELISA format, a stopping solution (such as sulfuric acid) is typically added to a TMB-containing solution to prevent extensive colour generation. After addition of the stop solution, the TMB solution changes from blue through to yellow (450 nm), generating a product that yields a high molar absorptivity. TMB chromogenic substrate is selected for its high sensitivity and stability.
    
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- Properties
  - Categories
    
    Benzamides and Anilines
    
    Alternative Name
    
    TMB;3,3,5,5-Tetramethylbenzidine
    
    CAS Number
    
    54827-17-7
    
    Molecular Formula
    
    C16H20N2
    
    Molecular Weight
    
    240.34 g/mol
    
    MDL Number
    
    MFCD00007748
    
    Note
    
    There is no hazardous surcharge associated with this product.
    There is no special packaging charge associated with this product.
    
    * For research use only. Not for human or veterinary use
- Reference
  - Han., et al. (2018). Convenient and Sensitive Colorimetric Detection of Melamine in Dairy Products Based on Cu(ii)-H2O2-3,3,5,5-tetramethylbenzidine System. RSC Advances 8(61):34877–34882
    Portsmann., et al. (1991). Tetramethylbenzidine—A Chromogenic Substrate for Peroxidase in Enzyme Immunoassay. Zeitschrift für Medizinische Laboratoriumsdiagnostik 32(1):3–8