Hifair® IV Reverse Transcriptase (200 U/μL)

Hifair™ IV Reverse Transcriptase is an updated version of Hifair™ Ⅱ Reverse Transcriptase obtained through genetic engineering technology. It has higher cDNA synthesis ability and speed, thermal stability and reaction temperature limit (up to 60°C) than Hifair™ Ⅱ Reverse Transcriptase. The synthesized cDNA product is up to 19.8 kb. Hifair™ IV Reverse Transcriptase enhances the affinity of the templates and is suitable for reverse transcription of RNA templates with complex secondary structure or low copy genes.

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using Oligo(dT) as primers.

Components: 5×HifairTM IV Buffer (250 μL/1,250 μL); HifairTM IV Reverse Transcriptase (200 U/μL) (50 μL/250 μL)

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