WST-8 Cell Proliferation Kit -for Transfection Assay

- Overview
  - The WST-8 Cell Proliferation Kit is a colorimetric assay for the determination of viable cell numberand for studying induction or inhibition of cell proliferation in vitro.
    
    Main Features
    More sensitive than other formazan-based assays
    Colorimetric measurement at 450 nmallows quantification of viable cells. Large linearity range independent from temperature, pH or concentration
    Allow to perform real-time assays
    Rapid and ready-to-use. One step procedure / One reagent only. No need of washing or extraction procedures,no other solvent or solubilisation solution required
    Highly reproducible. Adaptable from 384-well plates for screening to larger cell culture formats / Perfect for High Throughput Screening
    Suitable for adherent as well as suspension cells. Compatible with cell lines, primary cells, bacteria…
    Final compound is not harmful for the cell
    
    Kit content
    10 mL of WST-8 reagent-For up to 1000 assays in 96-well plate
    
    Please contact us at for specific academic pricing.
- Properties
  - Storage
    
    Upon reception, store the WST-8 Cell Proliferation Kit at 4°C. Protect from light. Stability: 1 year at 4°CShipping Conditions: Shipped at RT
    
    Shipping
    
    Shipped at RT
- Applications
  - Application Description
    
    RECOMMENDED APPLICATIONS:
    For determination of viable cell number and for studying induction or inhibition of cell proliferation in vitro.
    As opposed to MTT assay, no solubilisation process is required since this formazan does not require solvation: the WST-8 is soluble in the tissue culture medium.
    The WST-8 Cell Proliferation Kit is more sensitive at neutral pH than other tetrazolium salts such as MTT, XTT, MTS or WST-1 and the amount of formazan produced is directly proportional to the number of living cells.
    Figure a: WST- 8 Cell Proliferation Kit mechanism: slightly yellow WST-8 is transformed into Yellow/Orange Formazan.
    This assay kit is based on the cellular reduction of the tetrazolium salt WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5- (2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] into a highly water-soluble, orange-colored formazan dye upon reduction in the presence of an electron carrier.

Documents

WST-8 Protocol

WST-8 MSDS

WST-8 Rapid Protocol

WST-8 Cell Proliferation Kit -for Transfection Assay

WST-8 Cell Proliferation Kit -for Transfection Assay

Documents

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