Nitric Oxide Detection Kit -for Transfection Assay

The Nitric Oxide Detection Kit is a colorimetric assay for the indirect determination of Nitric Oxide concentrations based on the enzymatic conversion of nitrate to nitrite by a nitrate reductase. NO are important physiological messengers and effector molecules involved in many physiological processes such as vasodilation, inflammation, thrombosis or immunity (…). This assay is based on the reaction originally described by Griess to detect NO2– in a variety of biological fluids such as cell culture medium, plasma, serum, or urine.

Main Features
Highly Sensitive: limit of detection ~2µM – 125 pmol
Colorimetric measurement at540-570 nm
Detection of NO2- in a linear and stable way

Kit content
For up to 500 assays in 96-well plate
25 mL reagent 1 (sulf.)25 mL reagent 2 (NED)1 mL Nitrite Standard (0.1 M)

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