Login / Register

0

Figure 1. Validation of the VIP Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the VIP Receptor 2 (VPAC2) were lysed and immunoblotted with the phosphorylation-independent anti-VPAC2 antibody (7TM0372N) at a dilution of 1:1000.

Figure 2. Immunocytochemical identification of VIP Receptor 2 in HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the VIP receptor 2 (VPAC2) were immunocytochemically stained with anti-VPAC2 (VIP receptor 2) antibody (7TM0372N) at a dilution of 1:200. Note, VPAC2 receptors were confined to the plasma membrane in transfected cells.

Figure 3. Immunohistochemical identification of VIP Receptor 2 in small intestine. Sections were dewaxed, microwaved in citric acid, and incubated with anti-VPAC2 (VIP receptor 2) antibody (7TM0372N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, VPAC2 receptors were detected at the plasma membrane of neuroendocrine cells in small intestine.

Figure 4. Immunohistochemical identification of VIP Receptor 2 in breast carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-VPAC2 (VIP receptor 2) antibody (7TM0372N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, VPAC2 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.

VPAC2 ( non-phospho ), VIP Receptor 2 Polyclonal Antibody

VPAC2 ( non-phospho ), VIP Receptor 2 Polyclonal Antibody

Catalog Number:

A001455968TMA

Mfr. No.:

7TM0372N

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The VPAC2 antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human VIP receptor 2. It can be used to detect total VPAC2 receptors in Western blots independent of phosphorylation. The VPAC2 antibody can also be used to isolate and enrich VPAC2 receptors from cell and tissue lysates. It also detects VPAC2 in cultured cells and tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  A synthetic peptide with sequence LQFHRGSRAQSFLQTETSVI corresponds to amino acids 419-438 of VPAC2.
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  VPAC2, VIP Receptor 2, Vasoactive Intestinal Peptide Receptor 2 (UniProt:P41587 (human), P41588 (mouse), P35000 (rat))
  
  * This product is intended for research use only.

Applications

Application

WB; ICC; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100

Reference
- Schulz S, Röcken C, Mawrin C, Weise W, Höllt V, Schulz S. Immunocytochemical identification of VPAC1, VPAC2, and PAC1 receptors in normal and neoplastic human tissues with subtype-specific antibodies. Clin Cancer Res. 2004 Dec 15;10(24):8235-42.
  Schulz S, Mann A, Novakhov B, Piggins HD, Lupp A. VPAC2 receptor expression in human normal and neoplastic tissues: evaluation of the novel MAB SP235. Endocr Connect. 2015 Mar;4(1):18-26. doi: 10.1530/EC-14-0051. Epub 2014 Dec 11. PubMed PMID: 25504760; PubMed Central PMCID: PMC4285768.

Note: If you don't receive our verification email, do the following:

Confirm that you entered your email address correctly.
Check if the email is in your spam or junk folder.
Or you may contact us at .

Products

Amerigo Scientific

Support

Contact Us

Copyright © Amerigo Scientific. All rights reserved.