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Figure 1. Validation of the VIP receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the VIP Receptor 1 (VPAC1) were lysed and immunoblotted with the anti-VPAC1 antibody (7TM0371N) at a dilution of 1:1000.

Figure 2. Immunocytochemical identification of VIP Receptor 1 in HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the VIP Receptor 1 (VPAC1) were immunocytochemically stained with anti-VPAC1 (VIP Receptor 1) antibody (7TM0371N) at a dilution of 1:200. Note, VPAC1 receptors were confined to the plasma membrane in transfected cells.

Figure 3. Immunohistochemical identification of VIP Receptor 1 in small intestine. Sections were dewaxed, microwaved in citric acid, and incubated with anti-VPAC1 (VIP Receptor 1) antibody (7TM0371N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, VPAC1 receptors were uniformly detected at plasma membrane of eptihelial cells.

Figure 4. Immunohistochemical identification of VIP Receptor 1 in small intestine. Sections were dewaxed, microwaved in citric acid, and incubated with anti-VPAC1 (VIP Receptor 1) antibody (7TM0371N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, VPAC1 receptor staining is absent in muscosa but located in basal portion of the crypts.

Figure 5. Immunohistochemical identification of VIP Receptor 1 in enteric ganglia. Sections were dewaxed, microwaved in citric acid, and incubated with anti-VPAC1 (VIP Receptor 1) antibody (7TM0371N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, VPAC1 receptors were uniformly detected in nearly all cells of enteric ganglia.

VPAC1 ( non-phospho ), VIP Receptor 1 Polyclonal Antibody

VPAC1 ( non-phospho ), VIP Receptor 1 Polyclonal Antibody

Catalog Number:

A001455969TMA

Mfr. No.:

7TM0371N

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The VPAC1 antibody is directed against the distal end of the carboxyl-terminal tail of human VIP Receptor 1. It can be used to detect total VPAC1 receptors in Western blots independent of phosphorylation. The VPAC1 antibody can also be used to isolate and enrich VPAC1 receptors from cell and tissue lysates. It also detects VPAC1 in cultured cells and tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human
  
  Immunogen
  
  A synthetic peptide with sequence TRVSPGARRSSSFQAEVSLV corresponds to amino acids 438-457 of human VPAC1.
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  VPAC1, VIP Receptor 1, Vasoactive Intestinal Peptide Receptor 1 (UniProt:P32241)
  
  * This product is intended for research use only.

Applications

Application

WB; ICC; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100

Reference
- Schulz S, Röcken C, Mawrin C, Weise W, Höllt V, Schulz S. Immunocytochemical identification of VPAC1, VPAC2, and PAC1 receptors in normal and neoplastic human tissues with subtype-specific antibodies. Clin Cancer Res. 2004 Dec 15;10(24):8235-42. PubMed PMID: 15623599.

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