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Overview
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SpCas9 is a DNA endonuclease guided by crRNA and tracrRNA (or sgRNA alone), which is derived from S. pyogenes. SpCas9 specifically recognizes and cleaves dsDNA target in a PAM (NGG)-dependent manner. The cleavage site of Cas9 in the target sequence is 3 bps away from the PAM site. In addition, SpCas9 can also specifically cleave ssDNA or ssRNA in the presence of DNA PAMmer sequences (for details, please refer to PMID: 24476820; PMID: 25274302). SpCas9 can also be used in in vitro experiments for cleaving target DNA and cloning target fragments (for details, please refer to PMID: 26323354).
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