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Overview
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SpCas9 is a DNA endonuclease guided by crRNA and tracrRNA (or sgRNA alone), which is derived from S. pyogenes. SpCas9 specifically recognizes and cleaves dsDNA target in a PAM (NGG)-dependent manner. The cleavage site of Cas9 in the target sequence is 3 bps away from the PAM site. The HNH and RuvC domains of SpCas9 are responsible for cleaving the target and non-target strands of dsDNA, respectively. In this product, the H840 residue in Cas9 is mutated to A, thereby inactivating its HNH domain. Therefore, only the RuvC domain in SpCas9 H840A Nickase (also called H840A nCas9) has the cleavage activity, cleaving the NT strand in the dsDNA target to generate a nicked dsDNA product.
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