uMRT Reverse Transcriptase

UltraMarathonRT™ Reverse Transcriptase Kit is designed to meet the evolving needs of the RNA research community. It is an ultra-processive reverse transcriptase that uses a single pass to create cDNAs from any type of RNA, including long, highly structured templates. Importantly, uMRT works optimally at ambient temperatures (20-42 ºC) removing any need to heat the RT reaction which preserves the integrity of your RNA samples.

Used for gene expression, RT-PCR, qRT-PCR, and other first strand cDNA synthesis applications with RNA input as low as 0.1 pg.

Reagents required but not supplied:
• dNTP mix stock (10 mM each)
The dNTP mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM. High purity nucleotides (>99%) are recommended, and the pH should be adjusted to 7.0 with sodium hydroxide.
• Oligo-dT primer, random primer or gene specific primer
• RNA template: 1 pg-2 μg total cellular RNA or 1 pg-500 ng of mRNA are recommended for each reaction.

Please contact us at for specific academic pricing.