MarathonRT Reverse Transcriptase

MarathonRT™ purified from Eubacterium rectale is a first generation, processive reverse transcriptase used to create cDNA from any RNA template.

Highlights:
Copies transcripts at least 10 kb in length and displays superior intrinsic RT processivity.
Low error frequency in single-molecule sequencing, comparable to that of other high-performance RTs.

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Background

MarathonRT is encoded within a eubacterial group II intron, and it has been shown to efficiently copy highly structured long RNA molecules in a single pass. MarathonRT can be used as a tool enzyme in a variety of applications that include single-cycle reverse transcription of long RNAs, dimethyl sulfate mutational profiling (DMS-MaP), selective 2'-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP), using ultra-long amplicons and the detection of natural RNA base modifications.