Trimethoprim ReadyMade™ Solution

- Overview
  - Trimethoprim ReadyMadeTM solution is provided as a sterile-filtered solution of Trimethoprim formulated in DMSO at a concentration of 25 mg/ml. It has been filter-sterilized using a 0.2 μm filter.
    Trimethoprim is a synthetic derivative of trimethoxybenzyl-pyrimidine with bacteriostatic and antiprotozoal properties. As a dihydrofolate reductase inhibitor, trimethoprim binds tightly to the bacterial enzyme, blocking the production of tetrahydrofolic acid from dihydrofolic acid, arresting folic acid synthesis.
    When Trimethroprim is combined with sulfonamides, like Sulfamethoxazole (S045), the two compounds show bactericidal effects, but are only bacteriostatic when used separately. The activity is attributed to their synergistic effect in inhibiting folic acid metabolism in bacteria.
    It is used against the bacteria causing recurrent cystitis, mild acute prostatitis, urinary tract infections, asymptomatic bacteriuria during pregnancy and respiratory tract infections.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Trimethoprim inhibits bacterial dihydrofolate reductase (DHFR) thus interfering with the conversion of dihydrofolate (DHF) to tetrahydrofolate (THF). THF is a precursor in the thymidine synthesis pathway and a disruption in this pathway inhibits bacterial the synthesis of bacterial DNA.
- Properties
  - CAS Number
    
    738-70-5
    
    Molecular Formula
    
    C14H18N4O3
    
    Molecular Weight
    
    290.32
    
    Other Properties
    
    Source: Synthetic
    
    Storage
    
    -20°C ; Protect from light
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Trimethoprim has a wide antibacterial spectrum and is active against most Gram-positive and Gram-negative aerobic bacteria, including Nocardia, Brucella, Gram-negative bacilli, and some Gram-positive bacteria like Streptococcus.
    
    Microbiology Applications: Trimethoprim is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC values include:
    Escherichia coli 0.25 µg/mL - 64 µg/mLHaemophilus influenzae 0.15 µg/mL - 16 µg/mL
    Media Supplement
    Trimethoprim can be used as a selective agent in several types of isolation media:
    Columbia Blood Agar - Campylobacter Selective Supplement (Skirrow)
    Columbia Blood Agar - Campylobacter Selective Supplement (Blaser-Wang)
    Campylobacter Agar - Campylobacter Selective Supplement (Preston)
    Columbia Blood Agar - Helicobacter pylori Selective Supplement (Dent)
    Bolton Broth - Bolton Broth Selective Supplement
    Campylobacter Agar Base - Modified Preston Campylobacter Selective Supplement
    Bolton Broth - Modified Bolton Broth Selective Supplement
    Chromogenic Bacillus cereus Agar - Chromogenic Bacillus cereus Selective Supplement