Tissue From Mice With SLE

Snap frozen tissue from mouse strains that spontaneously develop disease similar to systemic lupus erythematosus (SLE).

Analyze gene expression, protein presence, and biomarkers in tissue without a vivarium
Save time aging mice as disease develops; tissue is available from stock with no lead time.

Control tissues available for comparison
Tissue from young mice (prior to proteinuria development) - negative control for disease
Tissue from cyclophosphamide-treated mice - positive control for treatment

Tissue from (NZB x NZW)F1 mice
From female (NZB x NZW)F1 hybrid (NZBWF1) mice at 15 to 52 weeks of age.

These crossbred mice spontaneously develop a polygenic autoimmune disease closely resembling systemic lupus erythematosus (SLE) in human patients. Anti-nuclear and anti-dsDNA antibodies are detectable as early as 20 weeks of age. Proteinuria and kidney pathology (immune complex glomerulonephritis) are observed at 36 to 45 weeks.

Availability
Cyclophosphamide-treated (NZB x NZW)F1 hybrid (NZBWF1) mice with no or mild proteinuria (< 30 mg protein/dL).
Vehicle-treated or naïve (NZB x NZW)F1 hybrid (NZBWF1) mice with severe proteinuria (> 500 mg protein/dL).
Naïve (NZB x NZW)F1 hybrid (NZBWF1) mice, 15 to 18 weeks old, with no or mild proteinuria (< 30 mg protein/dL).

Tissue from MRL/lpr mice
From female MRL/lpr mice at 6 to 22 weeks of age.

MRL/lpr mice are homozygous for spontaneous mutation in the Fas gene, and develop lymphadenopathy (enlarged lymph nodes), splenomegaly (enlarged spleen) and systemic autoimmunity.

Autoimmune responses occur spontaneously in these mice, resulting in arthritis and glomerulonephritis resembling SLE in human patients. Anti-nuclear and anti-dsDNA antibodies are detectable as early as 8 weeks of age. Proteinuria and kidney pathology (immune complex glomerulonephritis) are found at 16 to 28 weeks of age.
Cyclophosphamide-treated MRL/lpr mice with no or mild proteinuria (< 30 mg protein/dL).

Vehicle-treated or naïve MRL/lpr mice with severe proteinuria (> 500 mg protein/dL).

Naïve MRL/lpr mice, 6 to 8 weeks old, with no or mild proteinuria (< 30 mg protein/dL).

Preparation
Blood is immediately collected for serum, organs are collected and snap frozen in liquid nitrogen. Serum is isolated and frozen at -80 °C.

All tissue is stored at -80 °C until shipment.

Please contact us at for specific academic pricing.