Tissue from Mice with EAE - snap frozen

Snap frozen tissue, fresh cells, and frozen serum from naïve mice (no immunization) and control mice (immunized with adjuvant only).

These products are for use as negative controls, for comparison to disease-induced mice. Tissue is time-matched to tissue from diseased mice.

Snap frozen tissue from mice immunized with adjuvant only (control kit; no CIA induction), matched for time from CIA induction.

Frozen serum from mice immunized with adjuvant only (control kit).

Preparation - frozen tissue
Female C57BL/6 or SJL mice 8 to 12 weeks of age are acclimated to our facility for at least 7 days.

In C57BL/6 mice, EAE is induced according to the protocol EAE Induction by Active Immunization in C57BL/6 Mice.

In SJL mice, EAE is induced according to the protocol EAE Induction by Active Immunization in SJL Mice.

Mice are scored daily for EAE severity on the scale of 0-5 in 0.5 unit increments (see protocol), and weighed 3 times/week (Monday, Wednesday, Friday).

Mice are euthanized at the appropriate stage of disease, immediately perfused with PBS, then spleen & spinal cord are isolated and snap frozen in liquid nitrogen.

Tissues are stored at -80 °C until shipment.

Please contact us at for specific academic pricing.