TF-1 Cell Lysate

- Overview
  - TF-1 cells have been derived from a patient with erythroleukaemia. TF-1 cells are completely dependent on GM-CSF or IL-3 for long term growth. TF-1 cells respond to a variety of other lymphokines and cytokines such as IL-1, IL-4, IL-6, IL-9, IL-11, IL-13, SCF, LIF and NGF. TF-1 cells do not express glycophorin A or carbonyl anhydrase I.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Cell Lysates
    
    Formulation
    
    Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
    
    Concentration
    
    2mg/ml (determined by BCA assay)
    
    Other Properties
    
    Quality Control: Western Blot analyzed by both GAPDH and Beta tubulin antibody
    
    Storage
    
    Aliquot the lysate into single tube and then storing them -80°C or below. Avoid repeated freezing and thawing cycles.
    
    * For research use only. Not intended for human, diagnostics or veterinary use.
- Applications
  - Application Description
    
    Heat lysate to 95°C for 5 minutes and rapidly cooling before use.