HepG2 Cell Lysate

- Overview
  - HepG2 cells are immortalized cell line that derived from liver tissue of a 15-year-old boy with hepatocellular carcinoma. HepG2 cells secrete a variety of major plasma proteins such as albumin, transferrin, and secrete acute-phase proteins such as fibrinogen, alpha 2-macroglobulin, alpha 1-antitrypsin, transferrin, and plasminogen. HepG2 have an epithelial morphology and are thought to be a very useful model to study HBV virus replication via transfection. Cells are also used for cancer and apoptosis studies.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Cell Lysates
    
    Formulation
    
    Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
    
    Concentration
    
    2mg/ml (determined by BCA assay)
    
    Other Properties
    
    Quality Control: Western Blot analyzed by both GAPDH and Beta tubulin antibody
    
    Storage
    
    Aliquot the lysate into single tube and then storing them -80°C or below. Avoid repeated freezing and thawing cycles.
    
    * For research use only. Not intended for human, diagnostics or veterinary use.
- Applications
  - Application Description
    
    Heat lysate to 95°C for 5 minutes and rapidly cooling before use.