PC-3 Cell Lysate

- Overview
  - PC3 cells are human prostate cancer cell lines that from a bone metastasis of a grade IV prostatic adenocarcinoma from a 62 year old male. These cells are useful in investigating the biochemical changes in advanced prostatic cancer cells and in assessing their response to chemotherapeutic agents. Moreover, they can be used to create subcutaneous tumors in mice in order to investigate a model of the tumor environment in the context of the organism.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Cell Lysates
    
    Formulation
    
    Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
    
    Concentration
    
    2mg/ml (determined by BCA assay)
    
    Other Properties
    
    Quality Control: Western Blot analyzed by both GAPDH and Beta tubulin antibody
    
    Storage
    
    Aliquot the lysate into single tube and then storing them -80°C or below. Avoid repeated freezing and thawing cycles.
    
    * For research use only. Not intended for human, diagnostics or veterinary use.
- Applications
  - Application Description
    
    Heat lysate to 95°C for 5 minutes and rapidly cooling before use.