T7 RNA Transcription Enzyme Mix, GMP Grade

As a biological macromolecule, mRNA can be synthesized on a large scale by in vitro transcription (IVT). T7 promoter is one of the promoters with the highest transcription efficiency. Therefore, T7 RNA Polymerase can be used for in vitro transcription to obtain more synthetic products. T7 RNA Transcription Enzyme Mix has been optimized by a series of transcription systems. One reaction can transcribe up to 150-200μg of RNA, and the synthesized RNA can be used downstream in many aspects such as mRNA vaccine preparation, RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection and in vitro translation application.
The original enzymes of T7 RNA Transcription Enzyme Mix produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.
This product has passed the HALAL certification.

Appearance: Clear and transparent solution
pH: 7.5-8.5
Performance: 1μl enzyme mix can be used to transcribe no less than 100μg RNA
Endonuclease Residues: The degradation of substrate was ≤10%
Exonuclease Residues: The degradation of substrate was ≤10%
RNase Residues: The degradation of substrate was ≤10%
Exogenous DNA Residues: ≤100pg/mg
Host-cell Protein Residues: ≤50ppm
Mycoplasma: Negative
Heavy Metals: ≤10ppm
Microbial Limit: Total aerobic microbial count≤1cfu/10ml, total yeasts and molds count≤1cfu/10ml

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