E. coli Poly(A) Polymerase, GMP Grade

E. coli Poly (A) Polymerase can catalyze the sequential incorporation of ATP into the 3´ end of RNA in the form of AMP independent of the presence of template. Poly(A) polymerase has high tailing efficiency and can add 20 to 200 A bases to the 3´ end of RNA. Polyadenylation improves the stability of the RNA in a cell and enhances the RNA expression efficiency after transfection or microinjection. RNAs with short poly(A) tails have a limited number of sites for oligo(dT) binding and will generate cDNA products of a uniform size, and RNAs with long poly(A) tails have multiple sites for binding the oligo(dT) primer/adapter and willgenerate cDNA products of heterogeneous size.

We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.

Appearance: Clear and transparent solution
Visible Particles: Meet the specification
pH: 7.5-8.5
Endonuclease Residues: The degradation of substrate was ≤10%
Exonuclease Residues: The degradation of substrate was ≤10%
Heavy Metal Residues: ≤10ppm
Microbial Limit: Total aerobic microbial count ≤1cfu/10ml, total yeasts and molds count≤1cfu/10ml
Activity Definition: At 37°C, within 10 mins, the amount of enzyme required to incorporate 1 nmol AMP into RNA within 10 min is defined as one unit of activity (U).

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