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Overview
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Sulfo-SMCC is a bifunctional reagent contaning a maleimide group and an N- hydroxysuccinimide active ester on different ends of the molecule. Maleimide group react with SH group, but N- hydroxysuccinimide active ester reacts specifically with amino groups. At pH above neutral, active ester group to form a stable amide bond with an amino group. On the other hand, maleimide groups can be reacted selectively with respect to the thiol group under the conditions of pH6-7. Further, Sulfo-SMCC having a cyclohexane structure linker site is characterized as compared with the same type of crosslinking agent having an aromatic ring in the linker portion, the stability of the maleimide groups that increase. Since the active ester group having a sulfonic acid group is introduced, it is possible to carry out the labeling reaction without using an organic solvent such as DMSO or DMF.
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- Properties
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Overview
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