BS3

- Overview
  - BS3 is a cross-linking reagent reacting the amino groups with each other. Since BS3 has at both ends of the molecule N-hydroxysuccinimide active ester to react selectively with the amino group, it can be conjugated a carrier protein to simple hapten or preparation labeled enzyme. BS3 takes a chemical structure having analkyl chain to the linker moiety. Meanwhile, DTSSP and DSP is capable of cleavage of the disulfide-introduced linker site that is easily reduced by the reducing agent. Further, since the active ester group having a sulfonic acid group is introduced to DTSSP and BS3, it may be carried out labeling reaction without using an organic solvent such as DMSO or DMF.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Cross-Linker
    
    Alternative Name
    
    Bis(sulfosuccinimidyl)suberate, disodium salt
    
    CAS Number
    
    82436-77-9(free acid)
    
    Molecular Weight
    
    C16H18N2Na2O14S2=572.43
    
    Appearance
    
    White to almost white powder
    
    Purity
    
    (HPLC): ≧93.0 %
    
    Storage
    
    0-5°C, Protect from moisture
    
    * For research use only
- Reference
  - 1) J. V. Staros, "N-Hydroxysulfosuccinimide Active Ester: Bis(N-hydroxysulfosuccinimide) Ester of Two Dicarboxylic Acids Are Hydrophilic, Membrane-impermeant, Protein Cross-Linkers", Biochemistry, 1982, 21, 3950.
    2) J. M. Salhany, R. L. Sloan and K. A. Cordes, "In Situ Cross-linking of Human Erythrocyte Band 3 by Bis8sulfosuccinimidyl)suberate", J. Biol. Chem., 1990, 265(29), 17688.
    3) J. E. Gestwicki, C. W. Cairo, L. E. Strong, K. A. Oetjen and L. L. Kiessling, "Influencing Receptor-Ligand Binding Mechanisms with Multivalent Ligand Architecture", J. Am. Chem. Soc., 2002, 124, 14922.
    4) S. S. Mark, N. Sandhyarani, C. Zhu, C. Campagnolo and C. A. Batt, "Dendrimer-functionalized Self-assembled Monolayers as a Surface Plasmon Resonance Sensor Surface", Langmuir, 2004, 20, 6808.

MSDS

BS3

We Also Recommend

DSP

DTSSP

Sulfo-AC5-SPDP