Sulfo-NHS-SS-Biotin

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Sulfo-NHS-SS-biotin(sulfosuccinimidyl-20(biotinamido)ethyl-1,3-dithiopropionate) is a long-chain cleavable amine-reactive biotinylation reagent. The presence of the negatively charged sulfonate group in the chemical structure of sulfo-NHS-SS-biotin makes it a water-soluble biotinylation reagent that can be directly added to aqueous reactions without prior dissolution of organic solvents. Although no prior dissolution is required, an aqueous stock solution of sulfo-NHS-SS-biotin must be prepared rapidly and used immediately in case of the occurrence of hydrolysis of the active ester. Sulfo-NHS-SS-biotin has been used to react with amine-containing proteins and other molecules forming a complex which further interacts with avidin or streptavidin probes and to purify targeted molecules using affinity chromatography on a column of immobilized avidin or streptavidin.
    Reference
    Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
- Properties
  - Categories
    
    Amine-reactive biotinylation reagent, mid-length
    
    Alternative Name
    
    Biotin disulfide N-hydroxysulfosuccinimide ester
    
    CAS Number
    
    325143-98-4
    
    Molecular Formula
    
    C19H27N4NaO9S4
    
    Molecular Weight
    
    606.7
    
    Purity
    
    98.00%
    
    Storage
    
    Store at -20°C. The product is not stable in solution, please dissolve it immediately before use.
    
    SMILES
    
    C1C(C(=O)N(C1=O)OC(=O)CCSSCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)[O-].[Na+]
    
    * For Research Use Only
- Reference
  - 1. Yang Y, Yu Q, et al. "Decreased NHE3 activity and trafficking in TGEV-infected IPEC-J2 cells via the SGLT1-mediated P38 MAPK/AKt2 pathway." Virus Res. 2020;280:197901. PMID:32070687
    2. Saladi S, Boos F, et al. "The NADH Dehydrogenase Nde1 Executes Cell Death after Integrating Signals from Metabolism and Proteostasis on the Mitochondrial Surface." Mol Cell. 2019 Oct 18. pii: S1097-2765(19)30732-4. PMID:31668496
    3. Sheridan J.S. Carrington. "Differential Glycosylation of the Inwardly Rectifying Potassium Channel Kir7.1 by G protein-coupled Receptors." Vanderbilt University.2019.
    4. Carrington S, Hernandez C, et al. "G protein-coupled receptors differentially regulate glycosylation and activity of the inwardly rectifying potassium channel Kir7.1." J Biol Chem. 2018 Sep 26. pii: jbc.RA118.003238. PMID:30257863
    5. Brasher MI, Martynowicz DM, et al. "Interaction of Munc18c and Syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumour cells." J Biol Chem. 2017 Aug 10. pii: jbc.M117.807438. PMID:28798239

Documents

COA (Certificate Of Analysis)

MSDS (Material Safety Data Sheet)

Datasheet

Protocol

Sulfo-NHS-SS-Biotin

Background

Documents

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