NHS-SS-Biotin

- Overview
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    Background
    
    NHS-SS-biotin (Succinimidyl-2-(biotinamido)-ethyl-1,3-dithiopropionate) is a long-chain cleavable amine-reactive biotinylation reagent that has a spacer arm of 24.3 A in length due to the existence of a cross-bridge within its chemical structure. The long spacer arm creates an increased length between a covalently modified molecule and the bicyclic biotin rings that greatly enhances the ability of the molecule to bind to avidin or streptavidin probes. The NHS ester group of NHS-SS-biotin reacts with the amine groups of proteins and other molecule forming an amide bond and releasing of sulfo-NHS. NHS-SS-Biotin is a water-insoluble molecule that requires the dissolution of organic solvents prior to be added to aqueous reactions.
    Reference
    Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
- Properties
  - Categories
    
    Amine-reactive biotinylation reagent, mid-length
    
    Alternative Name
    
    NHS-SS-Biotin,Biotin disulfide N-hydroxysuccinimide ester
    
    CAS Number
    
    122266-55-1
    
    Molecular Formula
    
    C19H28N4O6S3
    
    Molecular Weight
    
    504.64
    
    Purity
    
    98.00%
    
    Solubility
    
    ≥28.79 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH
    
    Storage
    
    Store at -20°C. The product is not stable in solution, please dissolve it immediately before use.
    
    SMILES
    
    O=C1CCC(N1OC(CCSSCCNC(CCCC[[email protected]@H]2SC[[email protected]@](N3)([H])[[email protected]]2([H])NC3=O)=O)=O)=O
    
    * For Research Use Only

Documents

COA (Certificate Of Analysis)

MSDS (Material Safety Data Sheet)

Datasheet

NHS-SS-Biotin

Background

Documents

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