Sulfo-NHS-LC-Biotin

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Sulfo-NHS-LC-biotin (Sulfosuccinimidyl 6-(biotinamido) Hexanoate) is an amine-reactive biotinylation agent. As a water-soluble analog of NHS-LC-biotin, Sulfo-NHS-LC-biotin contains a negatively charged sulfonate group on the NHS ring structure, which creates sufficient polarity within the molecule and allows it to be directly added into aqueous reactions without prior dissolution of organic solvents. Sulfo-NHS-LC-biotin covalently binds to amine groups in proteins and other molecules through its NHS ester forming an amide linkage and releasing N-Hydroxysuccinimide. Sulfo-NHS-LC-biotin has a greater length (22.4 A) between a covalently modified molecule and the bicyclic biotin rings than NHS-biotin (13.5 A), which provides the probes a greater opportunity to reach the binding sites deeply buried inside the surface plane of proteins.
    Reference
    Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
- Properties
  - Categories
    
    Amine-reactive biotinylation reagent, long chain
    
    Alternative Name
    
    Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt
    
    CAS Number
    
    127062-22-0
    
    Molecular Formula
    
    C20H29N4NaO9S2
    
    Molecular Weight
    
    556.6
    
    Purity
    
    98.00%
    
    Storage
    
    Store at -20°C. The product is not stable in solution, please dissolve it immediately before use.
    
    SMILES
    
    C1C(C(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)O.[Na]
    
    * For Research Use Only
- Reference
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    2. Jia J, Yue X, et al. "KDEL receptor is a cell surface receptor that cycles between the plasma membrane and the Golgi via clathrin-mediated transport carriers." Cell Mol Life Sci. 2020;10.1007/s00018-020-03570-3. PMID:32562023
    3. Wu Z, He D, et al. "A bimodal (SERS and colorimetric) aptasensor for the detection of Pseudomonas aeruginosa." Mikrochim Acta. 2018 Oct 31;185(11):528. PMID:30382404
    4. Shu F, Chen J, et al. "Cholesterol Crystal-Mediated Inflammation Is Driven by Plasma Membrane Destabilization." Front Immunol. 2018 May 29;9:1163. PMID:29896195
    5. Lorin, Charlotte, Isabelle Vögeli, and Ernst Niggli. "Dystrophic cardiomyopathy: role of TRPV2 channels in stretch-induced cell damage." Cardiovascular research 106.1 (2015): 153-162. PMID:25616416