Sulfo-HMCS - Amerigo Scientific

- Overview
  - Hetero-bifunctional cross-linking reagents have activated esters and maleimide reactive groups. These functional groups react with amines and sulfhydryl groups of proteins, respectively. Enzyme-labeled haptens are prepared using hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The cross-linking reaction requires neutral pH and mild temperature since it is necessary to maintain enzyme activity and antibody titers in the cross-linking reaction. Heterobifunctional cross-linking reagents with 3, 5, 7, or 10 linear carbon chains are available. These linear aliphatic chains act as spacers between the two reactive sites and their water-soluble reagents. They are more stable than the aromatic cross-linking reagents such as succinimidyl-4-N-maleimidobenzoate in a wider pH range.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Cross-Linking Reagents
    
    CAS Number
    
    211236-35-0
    
    Molecular Formula
    
    C16H19N2NaO9S
    
    Molecular Weight
    
    438.39
    
    Appearance
    
    white or slightly yellowish-pink powder
    
    Purity
    
    ≥90.0% (HPLC)
    
    Storage
    
    0-5°C, protect from moisture
    
    Shipping
    
    with blue ice
    
    * For research use only
- Reference
  - 1) J. V. Staros, N-Hydroxysulfosuccinimide Active Esters:Bis(N-hydroxysulfosuccinimide) Esters of Two Dicarboxylic acids Are Hydrophilic, Membraneimpermeant, Protein Cross-linkers, Biochemistry, 1982, 21, 3950.
    
    2) P. S. R. Anjaneyulu, James V. Staros, Reactions of N-Hydroxysulfosuccinimide Active Esters, Int. J. Peptide Protein Res., 1987, 30, 117.
    
    3) Y. Fukami, K. Sato, K. Ikeda, K. Kamisango, K. Koizumi and T. Matsuno, Evidence for Autoinhibitory Regulation of the c-src Gene Product a Possible Interaction Between the Src Homology 2 Domain and Autophosphorylation Site, J. Biol. Chem., 1993, 268, 1132.

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