STOP SOLUTION for TMB Substrate

The Stop solution is a sulphuric acid solution for colorimetric ELISA using TMB (3,3′,5,5′ – tetramethylbenzidine) substrate.
In the ELISA assays, in presence of HRP (horseradish peroxidase) enzyme conjugates, TMB and peroxide react to produce a blue colour. The colour intensity is proportional to the amount of HRP activity, which in turn is related to the levels of target analyte. Addition of Stop Solution changes the blue colour to yellow (absorbance maximum at 450 nm), stabilizing the colour development to enable accurate measurement of the intensity at 450 nm using a spectrophotometer or plate reader.

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