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Overview
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Recombinant full-length SpCas9 from S. pyogenes was expressed in E. coli using a C-terminal His tag. Cas9-NLS contains a nuclear localization signal for optimal activity. Enzyme supplied with 1 mL of Cas9 reaction buffer, 10x (CS09-09).
In bacteria and archaea, CRISPR-Cas systems provide sequence-based adaptive immunity against mobile genetic elements such as phages and plasmids (1). Cas9 is a multidomain nuclease from the bacterium Streptococcus pyogenes characteristic of type II CRISPR-Cas systems (2). Guided by a single-guide RNA (sgRNA), Cas9 targets specific genomic loci and generates a blunt-ended double-strand break (3). Cas9 can be used for genetic engineering experiments such as genome editing and generating transgenic models (4).
References:
: 1. Gong T, et al. 2019. CRISPR-Cas Systems in Streptococci. Curr Issues Mol Biol. 32:1-38.
2. Nishimasu H, et al. 2014. Crystal structure of Cas9 in complex with guide RNA and target DNA. Cell. 156(5):935-49.
3. Sapranauskas R, et al. 2011. The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli. Nucleic Acids Res.39(21):9275-82.
4. Adli M. 2018 The CRISPR tool kit for genome editing and beyond. Nat Commun. 9(1):1911.Please contact us at for specific academic pricing.
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Overview
SpCas9-NLS, Glycerol-Free (Purity: >90%)
SpCas9-NLS, Glycerol-Free (Purity: >90%)
Catalog Number:
E001578628SIG
Mfr. No.:
CS09N-E312HB-50; CS09N-E312HB-100
Price:
$262
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