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Overview
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Recombinant Thermus thermophilus protein TtCsm6 was expressed in E. coli cells using a C-terminal His tag.
TtCsm6 is an endoribonuclease specific for single-stranded RNA that consists of the N-terminal CARF domain and the C-terminal HEPN domain. The ribonuclease active site is formed by the HEPN domain dimer. TtCsm6 belongs to the Class 1 type-III-A CRISPR-Cas system (1). Upon activation of the CRISPR ancillary ribonucleases Csx1/Csm6, the HEPN active site degrades invasive RNA non-specifically. TtCsm6 is activated by the cyclic oligoadenylate second messengers that was converted from ATP by the Cas10 subunit of the Type III interference complex (2).
References:
1. Niewoehner, O. et al. 2016. Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6. RNA 22(3):318-329.
2. Niewoehner, O. et al. 2017. Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers. Nature 548(7669): 543-548.Please contact us at for specific academic pricing.
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Overview
TtCsm6 (Purity: >90%)
TtCsm6 (Purity: >90%)
Catalog Number:
E001578630SIG
Mfr. No.:
CS06-E311H-50; CS06-E311H-100
Price:
$317
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