SpCas9 (H840A)-NLS (Purity: >70%)

SpCas9 (H840A)-NLS (Purity: >70%)

Catalog Number:
E001578626SIG
Mfr. No.:
CS09N-E332H
Price:
$235
  • Size:
    Quantity:
    Add to Cart:
    Add to Cart
      • Overview

        • Recombinant full-length SpCas9 (H840A)-NLS from S. pyogenes expressed in E. coli using a C-terminal His tag. Contains a nuclear localization signal for optimal activity. Enzyme sold with 1 mL of Cas9 reaction buffer, 10x (CS09-09).
          Cas9 is a multidomain nuclease from the bacterium Streptococcus pyogenes characteristic of type II CRISPR-Cas systems (1). Guided by a single-guide RNA (sgRNA), Cas9 targets specific genomic loci and generates a blunt-ended double-strand break (2). SpCas9 (H840A)-NLS is a catalytic mutant of Cas9 containing a nuclear localization signal for optimal activity (3). Unlike the wild-type Cas9, SpCas9 (H840A)-NLS generates a single-strand DNA break on the non-targeting strand. SpCas9 (H840A)-NLS can be used for various genome editing applications.

          References:
          1. Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O. Crystal structure of Cas9 in complex with guide RNA and target DNA. 2014. Cell. 156(5):935-49.
          2. Sapranauskas R, Gasiunas G, Fremaux C, Barrangou R, Horvath P, Siksnys V. 2011. The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli. Nucleic Acids Res. 39(21):9275-82.
          3. Jiang F, Doudna JA. 2017. CRISPR-Cas9 Structures and Mechanisms. Annu Rev Biophys.46:505-529.

          Please contact us at for specific academic pricing.

      • Properties
        • Name
          Cas9-H840A Nickase Protein
          Source
          Streptococcus pyogenes
          Type
          CRISPR Enzymes
          Purification
          Purity>70%
          Formulation
          Protein stored in 50 mM sodium phosphate, pH 7.5, 300 mM NaCl, 200 mM imidazole, 1 mM DTT, 10% glycerol
          Storage
          Store at –70°C. To avoid repeated handling and multiple freeze/thaw cycles aliquot diluted product into smaller quantities.
          Concentration
          0.2 μg/μl
          6.26 pmol/μg
          Molecular Weight
          Calculated: 161.0 kDa
          Observed: ~160 kDa
          Expression system
          E. coli
          Sequence
          full length
          Affinity Tag
          His

          * For Research Use Only.

    Documents

    CS09N-E332H-SpCas9 (H840A)-NLS

    SDS-CS09N-E332H-SpCas9 (H840A)-NLS

    SDS-CS09-09-Cas9 Reaction Buffer, 10X

    We Also Recommend

    SpCas9-NLS (Purity: >70%)

    SpCas9-NLS (Purity: >70%)

    $262

    SpCas9-NLS, Glycerol-Free (Purity: >90%)

    SpCas9-NLS, Glycerol-Free (Purity: >90%)

    $262

    TccCas13a (Purity: >95%)

    TccCas13a (Purity: >95%)

    $317