MagPure Total RNA Kit

MagPure Total RNA Kit

Catalog Number:
NAPK357158ANG
Mfr. No.:
R662201; R662202; R662203
Price:
$403
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      • Overview
        • This product supplies a simple and rapid extraction of total RNA from tissue and culture cells samples. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction processis only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure LQ Kit buffers can be used for both manual extraction process and automatic nucleic acid extraction machines.

          Principle
          The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.

          Advantages
          • High purity - OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
          • Economy - less than 50% of the price of Qiagen and other imported products
          • Automatic - extraction without manual participation, saving time and effort
          • Universal - suitable for various animal tissues, cultured cells and conventional plant fungal tissues

          Please contact us at for specific academic pricing.

      • Properties
        • Details
          Purification method: Polydisperse magnetic beads
          Purification technology: Magnetic beads technology
          Process method: Manual or automatic
          Sample type: Animal tissue, human tissue, cultured cells, lymphocytes, ordinary plant tissue
          Sample amount: Cells: 1 x 107
          Animal tissue: ≤20mg
          Plant tissue: ≤100mg
          Yeast cell: ≤5 x 106
          Storage
          MagPure Particles, DNase I and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

          * For Research Use Only

      • Applications
        • Application
          RT-PCR, cDNA synthesis, second generation sequencing
          Application Description
          Isolation total RNA from tissue and cells and plant

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