Seplife® 6AG NTA/90 Affinity Chromatography Agarose Resin

Seplife® 6AG NTA/90 Affinity Chromatography Agarose Resin

Catalog Number:
CM01530313SUN
Mfr. No.:
A400302x
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      • Overview
        • Seplife® 6AG NTA/90 is a metal affinity chromatography resin based on a 6% highly crossed linked agarose functionalized with nitrilotriacetic acid (NTA).

          • Seplife® 6AG NTA/90 can be loaded with a range of divalent metals such as Ni, Co, Cu, Fe, Zn showing high affinity for His-tagged proteins.

          • Suitable for small scale and large scale purifications of His-tagged proteins from other proteins in the feed.

          • Seplife® 6AG NTA/90 resin provides high dynamic binding capacity toward His-tag proteins upon loading with divalent metal.

          • High stability to CIP (cleaning in place) up to 1.0M NaOH.

          • Regulatory Support File (RSF) documentation is available for Seplife® 6AG NTA/90

          Seplife® 6AG NTA/90 is based on highly cross-linked (6%) agarose functionalized with NTA and ready to be loaded with a divalent metal of choice for affinity binding of His-tag proteins and has a particle size in the range 45- 165 microns.

          • Can be loaded with a range of divalent metals such as Ni, Co, Cu, Fe, Zn showing high affinity for His-tagged proteins

          • Suitable for small scale and large scale purifications of His-tagged proteins

          High dynamic binding capacity CIP stability <1.0M NaOH
          Seplife® 6AG NTA/90 is a metal affinity chromatography resin based on a 6% highly crossed linked agarose functionalized with nitrilotriacetic acid (NTA).
          Seplife® 6AG NTA/90 is based on highly cross-linked (6%) agarose functionalized with NTA and ready to be loaded with a divalent metal of choice for affinity binding of His-tag proteins and has a particle size in the range 45-165 micron.

          Please contact us at for specific academic pricing.

          More Details

      • Properties
        • Categories
          Affinity Chromatography - Agarose
          Other Properties
          Appearance: White spherical beads
          Type: Metal affinity chromatography resin (NTA)
          Matrix: 6% cross-linked agarose
          Ligand: Nitrilotriacetic acid
          Particle size range (μm): 45-165
          pH stability: 3-13 (operational), 2-14 (CIP)
          Chemical Stability: Stable in all common aqueous buffers; 1M sodium hydroxide; 8M urea; 6M guanidine hydrochloride; 70% ethanol.
          Flow rate* (cm/h): max 750cm/h
          Shipped as: 20% ethanol slurry

          *Testing conditions: Chromatography column 16mm×200mm; column bed height 20cm; temperature 25°C; mobile phase water

          * For Research Use Only.

      • Applications
        • Application Description
          Sealed and stored at 4~30°C (preservation solution is 20% ethanol) in a ventilated, dry and clean place, do not freeze.

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