Name: rTrypsin-N Protease, 140u, Mass Spectra Grade
Price: 427.00 USD

- Overview
  - Mass Spectrometry-grade rTrypsin-N Protease specifically hydrolyzes peptide bonds at the N-terminus of lysine (K) and arginine (R) residues. It is a mirror-image protease of trypsin and belongs to the family of N-terminal–cleaving proteases.
    
    Physical Form: Mass spectrometry-grade rTrypsin-N protease is provided as a lyophilized powder.
    Reconstitution: Dissolve in 40 µL of ultrapure water (pH 7.5) before use.
    Shelf Life: 24 months when stored at –80°C.
    pH Range: rTrypsin-N exhibits optimal activity at pH 7.5.
    Application: rTrypsin-N specifically hydrolyzes peptide bonds at lysine (K) and arginine (R) sites of denatured proteins.
    
    General Solution Digestion Method:
    Recommended digestion buffer: 20–50 mM HEPES or Tris buffer.
    Digestion conditions: Use the enzyme at a protein-to-enzyme ratio of 20:1 (w/w). Incubate at 37°C for more than 4 hours.
    
    Cleavage Sites: R, K
    
    Please contact us at for specific academic pricing.
- Properties
  - Storage
    
    Lyophilized powder should be stored at –20°C. After reconstitution, the enzyme solution can be stored at –20°C or –80°C.
    
    Note
    
    Maximum Activity: For optimal activity, it is recommended that protein samples undergo denaturation and alkylation, and that the digestion reaction system and SOP are strictly followed.
    
    Sensitivity: The enzyme is sensitive to 1 mM DTT, 0.1 mM guanidine hydrochloride, 0.8 M urea, and 5% acetonitrile, which can reduce digestion efficiency.
    
    Molecular Weight
    
    29kDa.
    
    * For Research Use Only.
- Applications
  - Application Description
    
    For studies of R/K amino acid post-translational modifications (PTMs), such as neuroproteomics, Alzheimer's disease (AD) clinical diagnostics, and peptide drug characterization.

rTrypsin-N Protease, 140u, Mass Spectra Grade

rTrypsin-N Protease, 140u, Mass Spectra Grade

We Also Recommend

rAsp-N Protease, 1800u, Mass Spectra Grade

rGlu-C Protease, 800u, Mass Spectra Grade

rLys-N Protease, 400u, Mass Spectra Grade