ROS Detection Assay Kit -for Transfection Assay

TheROS Detection Assay kit (Green Fluorescence)is a cell-based assay designed for measuring Reactive Oxygen Species (ROS) activity within a cell using the cell-permeable fluorogenic probe 2’, 7’-Dichlorodihydrofluorescein diacetate (DCF-DA).
Once DCF-DA has diffused into cells it is deacetylated by cellular esterases to a non-fluorescent compound and rapidly oxidized by ROS into DCF. DCF is highly fluorescent and can be detected by microscopy, titration in microplate and also cytometry (ex 485/em 535). The fluorescence intensity that results is proportional to the ROS levels within the cell.

Main Features
Measure ROS production in live cells
Ideal for fluorescence microscopy, microplate titration and cytometry
Compatible with adherent cells as well as suspension cells
Kit content
For up to 300 assays in 96-well plate
25mM DFC-DA in DMF (35µL) -10X ROS Buffer (5 mL)
Tert-Butyl Hydrogen Peroxide (TBHP) 25 mM (50 µL)
Each ROS Kit provides sufficient reagents for approximately 300 measurements in microplate format and includes Tert-Butyl hydroperoxide (organic peroxide) solution provided as a positive control for ROS generation.

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