Name: ROS Detection Assay Kit (DCFDA / H2DCFDA)
Price: 514.00 USD

- Overview
  - ROS Detection Assay Kit is a highly sensitive and safe assay to measure Reactive Oxygen Species. ROS include a number of reactive molecules and free radicals, derived from molecular oxygen, that damage DNA and RNA and oxidize proteins and lipids (lipid peroxidation). The most common ROS include superoxide anion (O2•-), hydrogen peroxide (H2O2), hydroxyl radical (HO•) and singlet oxygen (1O2), all of which are more reactive than oxygen (O2) itself. The molecules are produced during the electron transport of mitochondrial aerobic respiration or by oxidoreductase enzymes and metal catalysed oxidation.
    
    Advantages & Features
    
    Based on unique fluorescent probe H2DCFDA.
    
    Fast & Easy Protocol.
    
    Safe: non-Radioactive, no special handling needed.
    
    High-Throughput.
    
    Accurate & Reproducible results.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Oxidative Stress Assay Kits
    
    Storage
    
    4 °C upon receipt. 24 months from the date of purchase, if it is properly stored.
    
    * This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals.
- Applications
  - Application Description
    
    Highly Sensitive & Safe Assay for Reactive Oxygen Species Measurement.
- Reference
  - López-Grueso, M. J., Lagal, D. J., García-Jiménez, Á. F., Tarradas, R. M., Carmona-Hidalgo, B., Peinado, J., … & Padilla, C. A. (2020). Knockout of PRDX6 induces mitochondrial dysfunction and cell cycle arrest at G2/M in HepG2 hepatocarcinoma cells. Redox Biology, 101737.
    
    Criel, H. (2019). INTRACELLULAR ACCUMULATION OF QUERCETIN AND ITS IMPACT ON CELLULAR STRESS: AN IN VITRO STUDY (Doctoral dissertation, Ghent University).
    
    Luparello, C., Asaro, D. M. L., Cruciata, I., Hassell-Hart, S., Sansook, S., Spencer, J., & Caradonna, F. (2019). Cytotoxic Activity of the Histone Deacetylase 3-Selective Inhibitor Pojamide on MDA-MB-231 Triple-Negative Breast Cancer Cells. International Journal of Molecular Sciences, 20(4), 804.

Datasheet

Documents

Manual

ROS Detection Assay Kit (DCFDA / H2DCFDA)

ROS Detection Assay Kit (DCFDA / H2DCFDA)

Documents

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