Recombinant Ten-Eleven Translocase (TET)

Recombinant Ten-Eleven Translocase (TET) is a highly efficient Fe2+ and α-ketoglutarate (α-KG)-dependent dioxygenase based on TET enzyme modification and recombinant expression. Through a series of oxidation reactions, 5-methylcytosine (5mC) can be converted into 5-hydroxymethylcytosine (5hmC), and then into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). 5caC is further converted into uracil (U) by the action of a converter or enzyme, and uracil (U) is converted into thymine (T) by PCR, thereby achieving single-base resolution and high-accuracy DNA methylation site identification.

Recombinant Ten-Eleven Translocase (TET) (5 μg/μL): 200 μL

Instructions:
EM-seq input ~40ug/T, the reaction temperature of the enzyme is 37°C, full reaction generally takes 60 min, and termination reaction is required.
The amount of enzyme input can be adjusted appropriately according to experimental requirements.

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