Hieff Clone™ Universal II One Step Cloning Kit

Hieff Clone™ Universal II One Step Cloning Kit is a new generation homologous recombinant cloning kit. The carefully optimized 2nd generation 2× Hieff Clone™ Universal II Enzyme Premix combines the recombinant enzyme and the buffer required for the recombinant reaction with the addition of a unique recombinant enhancer to significantly improve the efficiency of recombinant cloning.

The kit can be directed to clone PCR products to any site of any vector, compatible with unpurified PCR products, directly recovered PCR products, low concentration of rubber recovered products, this product can reassemble the homologous arm GC content of 30%-70% of the joint fragment. The vector was completely linearized, and homologous sequences of the end of the linearized vector of 15-25 bp were introduced into the 5 'end of the positive and reverse PCR primers of the inserted fragment, so that the 5' and 3 'ends of the PCR products of the inserted fragment had exactly the same sequence corresponding to the two ends of the linearized vector, respectively. Under the action of recombinant enzyme, the recombinant reaction of PCR product and linearized vector can be completed in as little as 5 min at 50°C. The positive rate of cloning can reach more than 95%.

Features
· Simple: Seamlessly assemble and clone up to six DNA fragments in a single reaction.
· Flexible: Design guidelines allow assembly into any vector of your choice.
· Efficient: Efficient for ligation of one to six fragments.

A total of 10 ng low-input single fragment was cloned into a 10 kb plasmid.
Six fragments (a total length of 7.6 kb) was cloned into a 11.6 kb plasmid.
A 4.5kb fragment was cloned into a 21 kb plasmid.

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