Recombinant Human Polymerase (RNA) III (DNA directed) Polypeptide H

Recombinant Human Polymerase (RNA) III (DNA directed) Polypeptide H

Catalog Number:
P001413724ABE
Mfr. No.:
Abe32-4479
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Size:
20 µg
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    • Overview

      • POLR3H Human Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containing 227 amino acids (1-204 a.a.) and having a molecular mass of 25.3kDa (Molecular size on SDS-PAGE will appear higher). POLR3H is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Polymerase (RNA) III (DNA directed) Polypeptide H(POLR3H) is a member of the eukaryotic RPB7/RPC8 RNA polymerase subunit family. POLR3H is DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA utilizing the 4 ribonucleoside triphosphates as substrates. POLR3H has a central role in sensing and curbing infection by intracellular bacteria and DNA viruses. POLR3H functions as nuclear and cytosolic DNA sensor which is involved in innate immune response.

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    • Properties
      • Protein Name
        DNA-directed RNA polymerase III subunit RPC8, RNA polymerase III subunit C8, DNA-directed RNA polymerase III subunit H, RNA polymerase III subunit 22.9 kDa subunit, RPC22.9, POLR3H, KIAA1665, RPC8.
        Source
        E. coli
        Type
        Recombinant Proteins
        Purification
        Greater than 90.0% as determined by SDS-PAGE.
        Formulation
        POLR3H protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM DTT.
        Storage
        Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

        * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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