Recombinant Human Polymerase (RNA) II (DNA directed) Polypeptide J

Recombinant Human Polymerase (RNA) II (DNA directed) Polypeptide J

Catalog Number:
P001413725ABE
Mfr. No.:
Abe32-4480
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Size:
10 µg
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    • Overview

      • POLR2J Human Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containing 140 amino acids (1-117 a.a) and having a molecular mass of 15.7kDa. POLR2J is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Polymerase (RNA) II (DNA directed) Polypeptide J (POLR2J) is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The POLR2J protein occurs as a heterodimer with another polymerase subunit; together they develop a core subassembly unit of the polymerase. POLR2J is ubiquitously expressed, having a high expression in the heart and skeletal muscle.

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    • Properties
      • Protein Name
        DNA-directed RNA polymerase II subunit RPB11-a, RNA polymerase II subunit B11-a, RPB11a, DNA-directed RNA polymerase II subunit J-1, RNA polymerase II 13.3 kDa subunit, POLR2J, POLR2J1, RPB11, RPB11m, hRPB14.
        Source
        E. coli
        Type
        Recombinant Proteins
        Purification
        Greater than 90.0% as determined by SDS-PAGE.
        Formulation
        POLR2J protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol, 5mM DTT and 2mM EDTA.
        Storage
        Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

        * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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