Name: Recombinant Human Fibronectin (OsrhFN)
Price: Inquiry

- Overview
  - Fibronectin (FN) is a particularly important and well-studied component of the extracellular matrix, and is known to play a key role in cell adhesion, growth, spreading, migration, differentiation and proliferation. Fn is a 220 kDa glycoprotein composed of 2 subunits bound via a disulfide bond. Currently, the Fn is purified from the plasma, which however is limited by the availability of supply. The the recombinant human fibronectin (OsrhFN) was expressed in the rice endosperm platform, which is animal component free and has high purity, and has been demonstrated has the same physical and chemical with the plasma derived Fn. OsrhFN provides a safety solution to replace the plasma derived FN.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    OryzHiExp - A Rice Endosperm Specific Expression Platform
    OryzHiExp platform uses rice endosperm cells to express various recombinant proteins and small peptides at high levels. Through this platform, innovative animal-free protein products with a wide range of applications can be produced, such as OsrHSA, OsrhTF, OsrhFN, and other recombinant growth factors.
    ADVANTAGES
    • Elimination of the potential risk of viral and other unknown contaminants
    • Avoid the quality changes from different batches, improve the repeatability of cellculture and experiment results
    • Regulatory compliant with well-defined components
    • Stable supply and manufacture capability at large scales to meet bulk processing needs
    • Simplify the downstream bio-processing or biological research
- Properties
  - Source
    
    Oryza sativa (rice)
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    More than 95% as determined by SDS-PAGE.
    
    Formulation
    
    It is purified from rice endosperm.
    
    Storage
    
    Stored at temperatures -20°C for at least 12 months. For the lyophilized OsrhFN, it is recommended to reconstitute in the sterile water or PBS with 1mg/ml concentration. The reconstituted OsrhFN liquid can be made in other aqueous buffer. Upon reconstitution OsrhFN should be stored below -20°C. Use as soon as possible after opening. Please avoid freeze-thaw cycles.
    
    Endotoxin
    
    Less than 0.5EU/ug
    
    * For research, laboratory and manufacture use only. Not intended for direct use on humans.
- Applications
  - Application Description
    
    OsrhFN is generally used in the concentration range of 1-5 micrograms per cm² of growth surface for attachment or at 5-50 micrograms per ml as a media additive. (Optimal working dilutions must be determined by end user.)

In the SDS-PAGE and SEC-HPLC purity analysis, the purity of OsrhFN is more than 95%.

Compared with the commercial rat tail collagen and BD plasma derived Fn, the OsrhFN showed a higher cell viability and desity in CHO-K1 serum-free cell culture.

The morphology and immunofluorescent staining showed that OsrhFN was effective for keeping viability of neuron cells.

Recombinant Human Fibronectin (OsrhFN)

Recombinant Human Fibronectin (OsrhFN)

Background

In the SDS-PAGE and SEC-HPLC purity analysis, the purity of OsrhFN is more than 95%.

Compared with the commercial rat tail collagen and BD plasma derived Fn, the OsrhFN showed a higher cell viability and desity in CHO-K1 serum-free cell culture.

The morphology and immunofluorescent staining showed that OsrhFN was effective for keeping viability of neuron cells.

Recombinant Human Fibronectin (OsrhFN)

Recombinant Human Fibronectin (OsrhFN)

Background

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