Recombinant Human FGF2 Growth Factor

- Overview
  - Recombinant Human FGF2 is a serum-free sustainable growth factor ready to support your life-changing research in stem cells, tissue engineering, organoids, and beyond.
    
    Produced in a bioreactor-free, green-certified lab, our purified Recombinant Human FGF2 is demonstrated to be a high-performing, bioactive replacement for commercially available FGF2.
    
    - SPR readout shows binding affinity constant of less than 1 nM on ligand-specific antibody, and approx. 5 nM on the receptor, confirming protein identity and functionality.
    - Cell proliferation studies on NIH-3T3 lines demonstrates FGF2 drives proliferation in cells in a dose-dependent manner (EC50 between 1-3 ng/ml) via the activation of MAPK/ERK pathways
    - Purity of over 95% verified by SDS-PAGE.
    - Quality and safety test results show the product is negative for mycoplasma contamination and below safety threshold (less than 1 EU/ug) for endotoxin.
    
    Comes as lyophilized powder. Research use only.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    Fibroblast growth factor basic; Heparin-binding growth factor 2 (HBGF2); Prostatropin
    
    Source
    
    Drosophila melanogaster
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    Verified by SDS PAGE to be >95% pure
    
    Formulation
    
    Lyophilized protein with 50 mM Tris pH 8, 150 mM NaCl and 1 mM EDTA
    
    Storage
    
    Store at -15°C to -25°C upon receipt
    
    Sequence
    
    >sp|P09038|FGF2_HUMAN
    MAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
    
    Endotoxin
    
    Below threshold of <1 EU/µg
    
    Reconstitution
    
    Gently tap down the vial to ensure that all lyophilisate is collected at the bottom of the vial. Reconstitute the product in PBS to at least 100 μg/mL by gently pipetting the solution down the sides of the vial. Avoid vigorous shaking that can cause foaming and protein denaturation. Keep on ice. Aliquot and store at 5 ± 3°C for up to 2 weeks or at < -20°C for up to 3 months. Avoid repeated freeze-thaw cycles by aliquoting reconstituted products.
    
    Activity
    
    Determined by proliferation of NIH-3T3 cells
    
    * For research use only. Not for diagnostic or therapeutic use.

Datasheet

MSDS

Documents

Performance Report - Recombinant Human FGF2

Recombinant Human FGF2 Growth Factor

Recombinant Human FGF2 Growth Factor

Documents

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